A-GLIADIN RELATED SYNTHETIC PEPTIDES AGGLUTINATE K 562 S CELLS AND AFFECT IN VITRO DEVELOPING FETAL RAT INTESTINE AND ATROPHIC COELIAC MUCOSA

Effects of cereal peptides on the in vitro morphogenesis of rat fetal intestine.

Immunogenicity Characterization of Two Ancient Wheat α-Gliadin Peptides Related to Coeliac Disease

The immunogenic potential of α-gliadin protein from two ancient wheats was studied with reference to coeliac disease. To this aim we investigated Graziella Ra® and Kamut® (the latter is considered an ancient relative of modern durum wheat) in comparison to four durum wheat accessions (Senatore Cappelli, Flaminio, Grazia and Svevo). ELISA and Western Blot analyses - carried out by two monoclonal...

Epithelial Transport of Immunogenic and Toxic Gliadin Peptides In Vitro

SCOPE Celiac disease is an autoimmune disorder caused by failure of oral tolerance against gluten in genetically predisposed individuals. The epithelial translocation of gluten-derived gliadin peptides is an important pathogenetic step; the underlying mechanisms, however, are poorly understood. Thus, we investigated the degradation and epithelial translocation of two different gliadin peptides,...

In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells

The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared fr...

Gliadin peptide specific intestinal T cells in coeliac disease.

espite its heterogeneous clinical appearance, coeliac disease has a remarkable uniform human leuco-cyte antigen (HLA) association. The majority of coeliac disease patients carry a certain variant of HLA-DQ2, the rest carry HLA-DQ8. 1 2 As the physiological role of the HLA system is to present pep-tide fragments of antigens to T cells, it would seem logical that the HLA-DQ2 and HLA-DQ8 molecules...